Article
New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses.
Journal of virological methods - 1 Jul 2017
Naguib Mahmoud M, El-Kady Magdy F, Lüschow Dörte, Hassan Kareem E, Arafa Abdel-Satar, El-Zanaty Ali, Hassan Mohamed K, Hafez Hafez M, Grund Christian, Harder Timm C
Abstract excerpt
In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and...
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