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The Triple Response Assay and Its Use to Characterize Ethylene Mutants in Arabidopsis.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Merchante Catharina, Stepanova Anna N

Abstract excerpt

Exposure of plants to ethylene results in drastic morphological changes. Seedlings germinated in the dark in the presence of saturating concentrations of ethylene display a characteristic phenotype known as the triple response. This phenotype is robust and easy to score. In Arabidopsis the triple response is usually evaluated at 3 days post germination in seedlings grown in the dark in rich media supplemented...

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PMID: 28293847
DOI: 10.1007/978-1-4939-6854-1_13

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