Article
NADPH:protochlorophyllide oxidoreductase B (PORB) action in Arabidopsis thaliana revisited through transgenic expression of engineered barley PORB mutant proteins.
Plant molecular biology - 1 May 2017
Buhr Frank, Lahroussi Abderrahim, Springer Armin, Rustgi Sachin, von Wettstein Diter, Reinbothe Christiane, Reinbothe Steffen
Abstract excerpt
NADPH:protochlorophyllide oxidoreductase (POR) is a key enzyme for the light-induced greening of etiolated angiosperm plants. It belongs to the 'RED' family of reductases, epimerases and dehydrogenases. All POR proteins characterized so far contain evolutionarily conserved cysteine residues implicated in protochlorophyllide (Pchlide)-binding and catalysis. cDNAs were constructed by site-directed mutagenesis that...
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