Article
Common dyes used to determine bacterial polysaccharides on agar are affected by medium acidification.
Canadian journal of microbiology - 1 Jun 2017
Hawkins Justin P, Geddes Barney A, Oresnik Ivan J
Abstract excerpt
In this work, we highlight effects of pH on bacterial phenotypes when using the bacteriological dyes Aniline blue, Congo red, and Calcofluor white to analyze polysaccharide production. A study of galactose catabolism in Sinorhizobium meliloti led to the isolation of a mutation in dgoK1, which was observed to overproduce exopolysaccharides when grown in the presence of galactose. When this mutant strain was...
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