Article
Isolation, Culture, and Immunostaining of Skeletal Muscle Myofibers from Wildtype and Nestin-GFP Mice as a Means to Analyze Satellite Cell.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Stuelsatz Pascal, Keire Paul, Yablonka-Reuveni Zipora
Abstract excerpt
Multinucleated myofibers, the functional contractile units of adult skeletal muscle, harbor mononuclear Pax7+ myogenic progenitors on their surface between the myofiber basal lamina and plasmalemma. These progenitors, known as satellite cells, are the primary myogenic stem cells in adult muscle. This chapter describes our laboratory protocols for isolating, culturing, and immunostaining intact myofibers from...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.