Article
A Simplified and Systematic Method to Isolate, Culture, and Characterize Multiple Types of Human Dental Stem Cells from a Single Tooth.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Bakkar Mohammed, Liu Younan, Fang Dongdong, Stegen Camille, Su Xinyun, Ramamoorthi Murali, Lin Li-Chieh, Kawasaki Takako, Makhoul Nicholas, Pham Huan, Sumita Yoshinori, Tran Simon D
Abstract excerpt
This chapter describes a simplified method that allows the systematic isolation of multiple types of dental stem cells such as dental pulp stem cells (DPSC), periodontal ligament stem cells (PDLSC), and stem cells of the apical papilla (SCAP) from a single tooth. Of specific interest is the modified laboratory approach to harvest/retrieve the dental pulp tissue by minimizing trauma to DPSC by continuous...
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