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Rapid detection of Enterovirus and Coxsackievirus A10 by a TaqMan based duplex one-step real time RT-PCR assay.

Molecular and cellular probes - 1 Jun 2017

Chen Jingfang, Zhang Rusheng, Ou Xinhua, Yao Dong, Huang Zheng, Li Linzhi, Sun Biancheng

Abstract excerpt

A TaqMan based duplex one-step real time RT-PCR (rRT-PCR) assay was developed for the rapid detection of Coxsackievirus A10 (CV-A10) and other enterovirus (EVs) in clinical samples. The assay was fully evaluated and found to be specific and sensitive. When applied in 115 clinical samples, a 100% diagnostic sensitivity in CV-A10 detection and 97.4% diagnostic sensitivity in other EVs were found.

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PMID: 28189797
DOI: 10.1016/j.mcp.2017.02.003

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