Article
Promoter Trapping and Deletion Analysis Show Arabidopsis thaliana APETALA2 Gene Promoter Is Bidirectional and Functions as a Pollen- and Ovule-Specific Promoter in the Reverse Orientation.
Applied biochemistry and biotechnology - 1 Aug 2017
Sharma Pooja, Kumar Vajinder, Singh Sunil Kumar, Thakur Shweta, Siwach Priyanka, Sreenivasulu Yelam, Srinivasan Ramamurthy, Bhat Shripad Ramachandra
Abstract excerpt
The Arabidopsis thaliana promoter trap mutant Bitrap-112 expressing green fluorescent protein (GFP) gene in the ovules was found to carry transferred DNA (T-DNA) insertion at -309 position of the APETALA2 (AP2) gene. Bitrap-112 line did not show phenotype associated with the AP2 mutation, suggesting that T-DNA insertion did not interrupt the AP2 promoter. Further, head-to-head orientation of GFP and AP2 genes...
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