Article
Optimisation and validation of a PCR for antigen receptor rearrangement (PARR) assay to detect clonality in canine lymphoid malignancies.
Veterinary immunology and immunopathology - 1 Dec 2016
Waugh Elspeth M, Gallagher Alice, Haining Hayley, Johnston Pamela E J, Marchesi Francesco, Jarrett Ruth F, Morris Joanna S
Abstract excerpt
PCR for antigen receptor gene rearrangements (PARR) analysis is being increasingly used to assist diagnosis of canine lymphoma. In this study, PARR was carried out on consecutive samples received as part of routine diagnostic practice from 271 patients: 195 with lymphoid malignancies, 53 with reactive conditions and 23 with other neoplasms. Initially, published primer sets were used but later minor primer...
Topics
- Animals
- DNA Primers
- Dog Diseases
- Dogs
- Female
- Gene Rearrangement
- Gene Rearrangement, B-Lymphocyte
- Gene Rearrangement, T-Lymphocyte
- Genotype
- Immunophenotyping
- Lymphoma
- Lymphoma, B-Cell
- Lymphoma, T-Cell