Article
Use of Combined MSAP and NGS Techniques to Identify Differentially Methylated Regions in Somaclones: A Case Study of Two Stable Somatic Wheat Mutants.
PloS one - 1 Jan 2016
Baránek Miroslav, Čechová Jana, Kovacs Tamas, Eichmeier Aleš, Wang Shunli, Raddová Jana, Nečas Tomáš, Ye Xingguo
Abstract excerpt
The appearance of somaclonal variability induced by in vitro cultivation is relatively frequent and can, in some cases, provide a valuable source of new genetic variation for crop improvement. The cause of this phenomenon remains unknown; however, there are a number of reports suggesting that epigenetics, including DNA methylations, are an important factor. In addition to the non-heritable DNA methylation changes...
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