Article
A cost-effective high-throughput metabarcoding approach powerful enough to genotype ~44 000 year-old rodent remains from Northern Africa.
Molecular ecology resources - 1 May 2017
Guimaraes S, Pruvost M, Daligault J, Stoetzel E, Bennett E A, Côté N M-L, Nicolas V, Lalis A, Denys C, Geigl E-M, Grange T
Abstract excerpt
We present a cost-effective metabarcoding approach, aMPlex Torrent, which relies on an improved multiplex PCR adapted to highly degraded DNA, combining barcoding and next-generation sequencing to simultaneously analyse many heterogeneous samples. We demonstrate the strength of these improvements by generating a phylochronology through the genotyping of ancient rodent remains from a Moroccan cave whose...
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