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First genotyping of Cryptosporidium spp. in pre-weaned calves, broiler chickens and children in Syria by PCR-RFLP analysis.

Veterinary parasitology - 30 Jul 2016

Kassouha Morshed, Soukkarieh Chadi, Alkhaled Abdulkarim

Abstract excerpt

In this study, PCR-RFLP was used for the first time in Syria for genotyping Cryptosporidium species of man, calves and chickens. The total of 391 fecal samples included 213 from children with diarrhea (<5years), 67 from pre-weaned calves with diarrhea and 111 from broiler chicken farms. All samples were collected and examined with acid fast stain to detect the positive samples. Subsequently a nested-PCR test was...

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PMID: 27369580
DOI: 10.1016/j.vetpar.2016.06.009

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