Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Optimized dissociation protocol for isolating human glioma stem cells from tumorspheres via fluorescence-activated cell sorting.

Cancer letters - 10 Jul 2016

Lv Donglai, Ma Qing-Hua, Duan Jiang-Jie, Wu Hai-Bo, Zhao Xi-Long, Yu Shi-Cang, Bian Xiu-Wu

Abstract excerpt

Fluorescence-activated cell sorting (FACS) based on the surface marker CD133 is the most common method for isolating glioma stem cells (GSCs) from heterogeneous glioma cell populations. Optimization of this method will have profound implications for the future of GSC research. Five commonly used digestion reagents, Liberase-TL, trypsin, TrypLE, Accutase, and non-enzymatic cell dissociation solution (NECDS), were...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to AC133 Antigen
Share to Cell Self Renewal

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Collagenases

Share to Multipotent Stem Cells

Share to Peptide Hydrolases

Share to Spheroids, Cellular

Share to Trypsin

Share to Tumor Burden

Source

Identifiers

PMID: 27091400
DOI: 10.1016/j.canlet.2016.04.022

Genes

GSC

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Optimized dissociation protocol for isolating human glioma stem cells from tumorspheres via fluorescence-activated cell sorting.PMID 27091400Read ↗

Current Strategies for Identification of Glioma Stem Cells: Adequate or Unsatisfactory?Semantic proximity Read ↗

Nanomedicines Targeting Glioma Stem CellsSemantic proximity Read ↗

Stem Cell Utilization for Potential Reduction in Glioblastoma Multiforme (GBM): A Focused Narrative ReviewSemantic proximity Read ↗

Advances in stem cell-based therapeutic transfers for glioblastoma treatmentSemantic proximity Read ↗

Marker-independent Method for Isolating Slow-Dividing Cancer Stem Cells in Human GlioblastomaSemantic proximity Read ↗

The Utility and Limitations of Neurosphere Assay, CD133 Immunophenotyping and Side Population Assay in Glioma Stem Cell ResearchSemantic proximity Read ↗

A novel approach to the identification and enrichment of cancer stem cells from a cultured human glioma cell lineSemantic proximity Read ↗

CD133-Positive Cells Might Be Responsible for Efficient Proliferation of Human Meningioma CellsSemantic proximity Read ↗

Targeting Glioblastoma Stem Cells: Cell Surface MarkersSemantic proximity Read ↗

Cancer stem cells from human glioma cell line are resistant to Fas-induced apoptosisSemantic proximity Read ↗

CD133 Is Essential for Glioblastoma Stem Cell MaintenanceSemantic proximity Read ↗

Isolation and characterization of cancer stem like cells in human glioblastoma cell linesSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.