Article
Three-Dimensional Imaging of Plant Organs Using a Simple and Rapid Transparency Technique.
Plant & cell physiology - 1 Mar 2016
Hasegawa Junko, Sakamoto Yuki, Nakagami Satoru, Aida Mitsuhiro, Sawa Shinichiro, Matsunaga Sachihiro
Abstract excerpt
Clearing techniques eliminate factors that interfere with microscopic observation, including light scattering and absorption by pigments and cytoplasmic components. The techniques allow fluorescence-based detailed analyses of materials and characterization of the three-dimensional structure of organs. We describe a simple and rapid clearing and imaging method, termed 'TOMEI' (Transparent plant Organ MEthod for...
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