Article
Genotyping of Trypanosoma cruzi DTUs and Trypanosoma rangeli genetic groups in experimentally infected Rhodnius prolixus by PCR-RFLP.
Acta tropica - 1 Apr 2016
Sá Amanda R N, Dias Greicy B M, Kimoto Karen Y, Steindel Mário, Grisard Edmundo C, Toledo Max Jean O, Gomes Mônica L
Abstract excerpt
The specific detection and genetic typing of trypanosomes that infect humans, mammalian reservoirs, and vectors is crucial for diagnosis and epidemiology. We utilized a PCR-RFLP assay that targeted subunit II of cytochrome oxidase and 24Sα-rDNA to simultaneously detect and discriminate six Trypanosoma cruzi discrete typing units (DTUs) and two genetic groups of Trypanosoma rangeli (KP1+/KP1-) in intestinal...
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