Article
[Genetic diversity of peanut bradyrhizobia estimated by culture-independent vs. culture-dependent approaches].
Wei sheng wu xue bao = Acta microbiologica Sinica - 4 Jun 2015
Chen Jingyu, Feng Yiguo, Wu Yanling, Meng Xiangfei, Hu Meijuan, Chen Wenfeng, Gu Jun
Abstract excerpt
OBJECTIVE: To develop a rapid technique for estimating the percentage of bradyrhizobial nodule occupancy, we comparaed the differences of genetic diversity of peanut bradyrhizobia with culture-independent and culture-dependent methods. METHODS: We used the traditional media plate technique for isolation of peanut bradyrhizobia and directly collected the bacteroids from peanut nodules. The BOX-PCR fingerprintings...
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