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Article

Identification of seven novel virulence genes from Xanthomonas citri subsp. citri by Tn5-based random mutagenesis.

Journal of microbiology (Seoul, Korea) - 1 May 2015

Song Xue, Guo Jing, Ma Wen-xiu, Ji Zhi-yuan, Zou Li-fang, Chen Gong-you, Zou Hua-song

Abstract excerpt

To identify novel virulence genes, a mutant library of Xanthomonas citri subsp. citri 29-1 was produced using EZ-Tn5 transposon and the mutants were inoculated into susceptible grapefruit. Forty mutants with altered virulence phenotypes were identified. Nine of the mutants showed a complete loss of citrus canker induction, and the other 31 mutants resulted in attenuated canker symptoms. Southern blot analysis...

Topics

Citrus paradisi
DNA Transposable Elements
Gene Library
Mutagenesis, Insertional
Mutation
Open Reading Frames
Phenotype
Plant Diseases
Plant Leaves
Real-Time Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Up-Regulation

Virulence

Xanthomonas

Source

Identifiers

PMID: 25935304
DOI: 10.1007/s12275-015-4589-3

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