Article
Species determination within Staphylococcus genus by extended PCR-restriction fragment length polymorphism of saoC gene.
FEMS microbiology letters - 1 Jan 2015
Bukowski Michal, Polakowska Klaudia, Ilczyszyn Weronika M, Sitarska Agnieszka, Nytko Kinga, Kosecka Maja, Miedzobrodzki Jacek, Dubin Adam, Wladyka Benedykt
Abstract excerpt
Genetic methods based on PCR-restriction fragment length polymorphism (RFLP) are widely used for microbial species determination. In this study, we present the application of saoC gene as an effective tool for species determination and within-species diversity analysis for Staphylococcus genus. The unique sequence diversity of saoC allows us to apply four restriction enzymes to obtain RFLP patterns, which appear...
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