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Article

A newly established real-time PCR for detection of Borrelia miyamotoi in Ixodes ricinus ticks.

Ticks and tick-borne diseases - 1 Apr 2015

Reiter Michael, Schötta Anna-Margarita, Müller Andreas, Stockinger Hannes, Stanek Gerold

Abstract excerpt

A total of 350 ticks collected in Austria were analyzed for the presence of DNA sequences of B. miyamotoi. Three ticks gave positive results in a B. miyamotoi-specific nested PCR. Results were confirmed by sequencing the amplified glpQ gene from the positive samples. Moreover we developed a real-time PCR which unambiguously detected B. miyamotoi in all positive samples. Further genotyping of the samples found...

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PMID: 25736476
DOI: 10.1016/j.ttbdis.2015.02.002

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