Article
Asparagine synthetase1, but not asparagine synthetase2, is responsible for the biosynthesis of asparagine following the supply of ammonium to rice roots.
Plant & cell physiology - 1 Apr 2015
Ohashi Miwa, Ishiyama Keiki, Kojima Soichi, Konishi Noriyuki, Nakano Kentaro, Kanno Keiichi, Hayakawa Toshihiko, Yamaya Tomoyuki
Abstract excerpt
Asparagine is synthesized from glutamine by the reaction of asparagine synthetase (AS) and is the major nitrogen form in both xylem and phloem sap in rice (Oryza sativa L.). There are two genes encoding AS, OsAS1 and OsAS2, in rice, but the functions of individual AS isoenzymes are largely unknown. Cell type- and NH4(+)-inducible expression of OsAS1 as well as analyses of knockout mutants were carried out in this...
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