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Article

Identification and validation of polymorphic microsatellite loci for the analysis of Phytophthora nicotianae populations.

Journal of microbiological methods - 1 Mar 2015

Biasi Antonio, Martin Frank, Schena Leonardo

Abstract excerpt

A large number of SSR loci were screened in the genomic assemblies of 14 different isolates of Phytophthora nicotianae and primers were developed for amplification of 17 markers distributed among different contigs. These loci were highly polymorphic and amplified from genetically distant isolates of the pathogen. Among these, nine were further validated using a multiplexed genotyping assay with differentially...

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PMID: 25601792
DOI: 10.1016/j.mimet.2015.01.012

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