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Article

Early detection of dengue virus by use of reverse transcription-recombinase polymerase amplification.

Journal of clinical microbiology - 1 Mar 2015

Teoh Boon-Teong, Sam Sing-Sin, Tan Kim-Kee, Danlami Mohammed Bashar, Shu Meng-Hooi, Johari Jefree, Hooi Poh-Sim, Brooks David, Piepenburg Olaf, Nentwich Oliver, Wilder-Smith Annelies, Franco Leticia, Tenorio Antonio, AbuBakar Sazaly

Abstract excerpt

A method for the rapid diagnosis of early dengue virus (DENV) infection is highly needed. Here, a prototype reverse transcription-recombinase polymerase amplification (RT-RPA) assay was developed. The assay detected DENV RNA in <20 min without the need for thermocycling amplification. The assay enabled the detection of as few as 10 copies of DENV RNA. The designed RT-RPA primers and exo probe detected the DENV...

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Cohort Studies
Dengue
Dengue Virus
Early Diagnosis
Molecular Diagnostic Techniques

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Reverse Transcriptase Polymerase Chain Reaction

Sensitivity and Specificity

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Identifiers

PMID: 25568438
DOI: 10.1128/JCM.02648-14
PMCID: PMC4390637

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