Article
Identification and validation of a new male sex-specific ISSR marker in pointed gourd (Trichosanthes dioica Roxb.).
TheScientificWorldJournal - 1 Jan 2014
Adhikari Sinchan, Saha Soumen, Bandyopadhyay Tapas Kumar, Ghosh Parthadeb
Abstract excerpt
The aim of the present study was to develop a genetic sex marker for the pointed gourd (Trichosanthes dioica Roxb.) to allow gender determination at any stage in the life cycle. Screening of genomic DNA with intersimple sequence repeat (ISSR) primers was used to discover sex-specific touch-down polymerase chain reaction (Td-PCR) amplification products. Using pooled DNA from male and female genotypes and 42 ISSR...
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