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De novo transcriptome assembly and the identification of gene-associated single-nucleotide polymorphism markers in Asian and American ginseng roots.

Molecular genetics and genomics : MGG - 1 Jun 2015

Jo Ick-Hyun, Lee Seung-Ho, Kim Young-Chang, Kim Dong-Hwi, Kim Hong-Sig, Kim Kee-Hong, Chung Jong-Wook, Bang Kyong-Hwan

Abstract excerpt

We performed de novo transcriptome sequencing for Panax ginseng and Panax quinquefolius accessions using the 454 GS FLX Titanium System and discovered annotation-based genome-wide single-nucleotide polymorphism (SNPs) using next-generation ginseng transcriptome data without reference genome sequence. The comprehensive transcriptome characterization with the mature roots of four ginseng accessions generated...

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PMID: 25527477
DOI: 10.1007/s00438-014-0974-6

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