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Multiplex RT-PCR and indirect immunofluorescence assays for detection and subtyping of human influenza virus in Tunisia.

Current microbiology - 1 Mar 2015

Ben M'hadheb Manel, Harrabi Myriam, Souii Amira, Jrad-Battikh Nadia, Gharbi Jawhar

Abstract excerpt

Influenza viruses are negative stranded segmented RNA viruses belonging to Orthomyxoviridae family. They are classified into three types A, B, and C. Type A influenza viruses are classified into subtypes according to the antigenic characters of the surface glycoproteins: hemagglutinin (H) and neuraminidase (N). The aim of the present study is to develop a fast and reliable multiplex RT-PCR technique for detecting...

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PMID: 25366277
DOI: 10.1007/s00284-014-0719-0

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