Article
Mutational definition of binding requirements of an hnRNP-like protein in Arabidopsis using fluorescence correlation spectroscopy.
Biochemical and biophysical research communications - 10 Oct 2014
Leder Verena, Lummer Martina, Tegeler Kathrin, Humpert Fabian, Lewinski Martin, Schüttpelz Mark, Staiger Dorothee
Abstract excerpt
Arabidopsis thaliana glycine-rich RNA binding protein 7 (AtGRP7) is part of a negative feedback loop through which it regulates alternative splicing and steady-state abundance of its pre-mRNA. Here we use fluorescence correlation spectroscopy to investigate the requirements for AtGRP7 binding to its intron using fluorescently-labelled synthetic oligonucleotides. By systematically introducing point mutations we...
Topics
Join the communities discussing this publication.