Article
Metal bridges illuminate transmembrane domain movements during gating of the cystic fibrosis transmembrane conductance regulator chloride channel.
The Journal of biological chemistry - 10 Oct 2014
El Hiani Yassine, Linsdell Paul
Abstract excerpt
Opening and closing of the cystic fibrosis transmembrane conductance regulator are controlled by ATP binding and hydrolysis by the cytoplasmic nucleotide-binding domains. Different conformational changes in the channel pore have been described during channel opening and closing; however, the relative importance of these changes to the process of gating the pore is not known. We have used patch clamp recording to...
Topics
- Animals
- CHO Cells
- Cadmium
- Cations, Divalent
- Chlorides
- Cricetulus
- Cysteine
- Cystic Fibrosis Transmembrane Conductance Regulator
- Gene Expression
- Ion Channel Gating
- Ion Transport
- Membrane Potentials
- Models, Molecular
- Mutation