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Article

PMS2 gene mutational analysis: direct cDNA sequencing to circumvent pseudogene interference.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014

Wimmer Katharina, Wernstedt Annekatrin

Abstract excerpt

The presence of highly homologous pseudocopies can compromise the mutation analysis of a gene of interest. In particular, when using PCR-based strategies, pseudogene co-amplification has to be effectively prevented. This is often achieved by using primers designed to be parental gene specific according to the reference sequence and by applying stringent PCR conditions. However, there are cases in which this...

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Source

Identifiers

PMID: 24823786
DOI: 10.1007/978-1-4939-0835-6_20

Genes

PMS2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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