[Stable green fluorescent protein expression in Toxoplasma gondii mutant].

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Article

[Stable green fluorescent protein expression in Toxoplasma gondii mutant].

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases - 1 Dec 2011

Wu Liang, Yuan Yi-Wei, Jiang Xu-Gan, Fu Xing-Li, Lu Zhao-Xi, Tu Guo-Hua, Li Li, Chen Sheng-Xia, Zhou Hong

Abstract excerpt

OBJECTIVE: To construct a Toxoplasma gondii mutant for stably expressing green fluorescent protein (GFP), and establish method to determine the rate of mutant-infected HeLa cells. METHODS: Freshly lysed-out tachyzoites of T. gondii RH strain were transfected with plasmid ptubP30-GFP/sag-CAT. Stable transformants were selected with chloramphenicol and limited dilution. The expression of GFP in mutant tachyzoite...

Topics

Female
Flow Cytometry
Fluorescence
Green Fluorescent Proteins
HeLa Cells
Humans
Mutation
Toxoplasma
Transfection

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PMID: 24822344

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