[Whole blood allele-specific PCR, a simple method to detect four ATP7B gene mutations in Wilson disease].

Abstract excerpt

OBJECTIVE: To establish a simple method to detect four ATP7B gene mutations in Wilson disease using allele-specific PCR(AS-PCR) with whole blood polymerase chain reaction. METHODS: Four allele-specific PCR primers specific for the mutations(G2333T, C2850T, G2855A, G2975T) were designed, and PCR was optimized to screen the whole blood samples. The amplified gene products with mutation were separated with agarose...

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