Article
Evaluation of a real-time PCR test for the detection and discrimination of theileria species in the African buffalo (Syncerus caffer).
PloS one - 1 Jan 2013
Chaisi Mamohale E, Janssens Michiel E, Vermeiren Lieve, Oosthuizen Marinda C, Collins Nicola E, Geysen Dirk
Abstract excerpt
A quantitative real-time PCR (qPCR) assay based on the cox III gene was evaluated for the simultaneous detection and discrimination of Theileria species in buffalo and cattle blood samples from South Africa and Mozambique using melting curve analysis. The results obtained were compared to those of the reverse line blot (RLB) hybridization assay for the simultaneous detection and differentiation of Theileria spp....
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