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Article

Detection and identification of genotypes of Prototheca zopfii in clinical samples by quantitative PCR analysis.

Japanese journal of infectious diseases - 1 Jan 2013

Onozaki Masanobu, Makimura Koichi, Satoh Kazuo, Hasegawa Atsuhiko

Abstract excerpt

In this study, a specific quantitative PCR system for the detection and identification of Prototheca zopfii genotypes was developed using a TaqMan(®) MGB probe and ResoLight dye. The P. zopfii-specific primers 18PZF1 and 18PZR1 were generated on the basis of the alignment of the small subunit ribosomal DNA domain base sequences of the genera Chlorella and Prototheca obtained from DDBJ/EMBL/GenBank, and the...

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Transition Temperature
DNA, Plant
DNA, Ribosomal
Mastitis, Bovine
Milk

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Diagnostic Techniques

Real-Time Polymerase Chain Reaction

Sensitivity and Specificity

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PMID: 24047735
DOI: 10.7883/yoken.66.383

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