Simultaneous detection of <i>Chlamydia</i> spp., <i>Coxiella burnetii</i> , and <i>Neospora caninum</i> in abortion material of ruminants by multiplex real-time polymerase chain reaction

Abstract excerpt

Chlamydia spp., Coxiella burnetii, and Neospora caninum are responsible for reproductive diseases and are closely linked with high abortion rates in ruminants. Furthermore, C. burnetii and Chlamydia spp. have zoonotic potential. A real-time polymerase chain reaction (PCR) assay was developed for the simultaneous detection of Chlamydia spp., C. burnetii, and N. caninum. The detection of beta-actin as internal...

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