Article
Conditioned medium from periapical follicle cells induces the odontogenic differentiation of stem cells from the apical papilla in vitro.
Journal of endodontics - 1 Aug 2013
Wu Jiayuan, Jia Qian, He Wenxi, Liu Jianguo, Hou Lipeng, Zhang Jing, Niu Zhongying, Ni Longxing
Abstract excerpt
INTRODUCTION: We investigated the biological effects of conditioned medium (CM) from periapical follicle cells (PAFCs) of root-developing tooth on the proliferation and differentiation of stem cells from the apical papilla (SCAP) in vitro. METHODS: Human SCAP and PAFCs were isolated and expanded. CM from PAFCs was prepared with the primary cells. Cell cycle analysis, methyl-thiazol-diphenyltetrazolium assay,...
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