Article
Optimization of DNA isolation and PCR protocol for analysis and evaluation of genetic diversity of the medicinal plant, Anemopsis californica using RAPD.
Acta biologica Hungarica - 1 Jun 2013
Lizette Del-Toro-Sánchez C, Villaseñor-Alvarado S, Zurita-Martínez Florentina, Castellanos-Hernández O A, Rodríguez-Sahagún Araceli, Isabel Torres-Morán M, Rojas-Bravo D, Gutiérrez-Lomelí M
Abstract excerpt
Anemopsis californica is a perennial herbaceous plant that has been utilized as a medicinal plant for the treatment of various diseases. The present work was carried out with the objective of optimizing a method of extraction of the genomic DNA of A. californica and a PCR protocol and later to evaluate the existing genetic diversity among the genotypes deriving from different origins. For DNA extraction, we...
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