Article
Molecular characterization of Cryptosporidium isolates from pre-weaned calves in western France in relation to age.
Veterinary parasitology - 18 Oct 2013
Rieux Anaïs, Paraud Carine, Pors Isabelle, Chartier Christophe
Abstract excerpt
Eighteen pre-weaned female calves from a single beef cattle herd in western France were sampled weekly from birth to 21/2 months of age in order to characterize Cryptosporidium oocyst output. 182 fecal samples were screened for the presence of oocysts after concentration using immunofluorescence analysis. DNA was extracted from positive samples and a PCR-RFLP protocol, with the restriction enzyme SspI and MboII,...
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