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Article

Optimization of PCR–RFLP Directly from the Skin and Nails in Cases of Dermatophytosis, Targeting the ITS and the 18S Ribosomal DNA Regions

1 Jan 2013

Abstract excerpt

PURPOSE: A pan fungal primer targeting the Internal Transcribed Spacer (ITS) region and optimization of PCR-RFLP using a dermatophyte specific primer targeted the 18S ribosomal DNA (rDNA) region were performed for the identification of dermatophyte species and strains directly from clinical specimens. MATERIALS AND METHODS: One hundred and thirty eight specimens (129 skin scrapings and 9 nail clippings) from...

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PMID: 23730638
DOI: 10.7860/jcdr/2013/5363.2873

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