Article
Routine clonal expansion of mesenchymal stem cells derived from amniotic fluid for perinatal applications
23 May 2013
Abstract excerpt
INTRODUCTION: Stem cells (SCs) isolated from amniotic fluid (AF) are a promising source for autologous perinatal cell therapy. The aim of this study was to develop a routine isolation, selection, and expansion protocol of clonal SC lines from redundant clinical amniocentesis samples. MATERIALS AND METHODS: Amniotic fluids were collected between 15 and 22 weeks of gestation, and SCs were isolated by CD117-based...
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