Development of a real-time RT-PCR assay for the simultaneous identification, quantitation and differentiation of avian metapneumovirus subtypes A and B

Abstract excerpt

In recent years, special attention has been paid to real-time polymerase chain reaction (PCR) for avian metapneumovirus (AMPV) diagnosis, due to its numerous advantages over classical PCR. A new multiplex quantitative real-time reverse transcription-PCR (qRT-PCR) with molecular beacon probe assay, designed to target the SH gene, was developed. The test was evaluated in terms of specificity, sensitivity and...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Respiratory viral infections research
• Share to Viral gastroenteritis research and epidemiology
• Share to Viral Infections and Immunology Research