Subgingival microbiome in smokers and non‐smokers in periodontitis: an exploratory study using traditional targeted techniques and a next‐generation sequencing

Abstract excerpt

AIM: To compare the results of two targeted techniques to an open-ended technique in periodontitis patients, differentiated on the basis of smoking habit. MATERIALS & METHODS: Thirty periodontitis patients (15 smokers and 15 non-smokers) provided subgingival plaque samples for 16S rRNA gene amplicon sequencing, culturing and quantitative polymerase chain reaction (qPCR). RESULTS: No differences were found in the...

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