Article
Barcoding of Giardia duodenalis isolates and derived lines from an established cryobank by a mutation scanning-based approach.
Electrophoresis - 1 Aug 2011
Nolan Matthew J, Jex Aaron R, Upcroft Jacqui A, Upcroft Peter, Gasser Robin B
Abstract excerpt
We barcoded 25 in vitro isolates (representing 92 samples) of Giardia duodenalis from humans and other animals, which have been assembled by the Upcroft team at the Queensland Institute of Medical Research over a period of almost three decades. We used mutation scanning-coupled sequencing of loci in the triosephosphate isomerase, glutamate dehydrogenase and β-giardin genes, combined with phylogenetic analysis, to...
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