Article
A newly developed real-time PCR assay for detection and quantification of Fusarium oxysporum and its use in compatible and incompatible interactions with grafted melon genotypes.
Phytopathology - 1 Aug 2013
Haegi Anita, Catalano Valentina, Luongo Laura, Vitale Salvatore, Scotton Michele, Ficcadenti Nadia, Belisario Alessandra
Abstract excerpt
A reliable and species-specific real-time quantitative polymerase chain reaction (qPCR) assay was developed for detection of the complex soilborne anamorphic fungus Fusarium oxysporum. The new primer pair, designed on the translation elongation factor 1-α gene with an amplicon of 142 bp, was highly specific to F. oxysporum without cross reactions with other Fusarium spp. The protocol was applied to grafted melon...
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