Article
cis-acting elements involved in replication of alfalfa mosaic virus RNAs in vitro.
Virology - 1 Jun 1990
van der Kuyl A C, Langereis K, Houwing C J, Jaspars E M, Bol J F
Abstract excerpt
A DNA copy of alfalfa mosaic virus (AIMV) RNA3 was transcribed in vitro in two different orientations with T7 RNA polymerase and the transcripts were used as templates for a virus-specific RNA-dependent RNA polymerase (RdRp) purified from AIMV-infected bean plants. Minus-stranded templates were t...
Topics
- Cloning, Molecular
- Enhancer Elements, Genetic
- Medicago sativa
- Mosaic Viruses
- Mutation
- Promoter Regions, Genetic
- RNA, Viral
- RNA-Dependent RNA Polymerase
- Regulatory Sequences, Nucleic Acid
- Restriction Mapping
- Templates, Genetic
- Transcription, Genetic
- Virus Replication