Article
Development of three PCR assays for the differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the co-endemic region of Qinghai-Tibet plateau, China.
The American journal of tropical medicine and hygiene - 1 Apr 2013
Boufana Belgees, Umhang Gérald, Qiu Jiamin, Chen Xingwang, Lahmar Samia, Boué Franck, Jenkins David, Craig Philip
Abstract excerpt
To investigate echinococcosis in co-endemic regions, three polymerase chain reaction (PCR) assays based on the amplification of a fragment within the NADH dehydrogenase subunit 1 (ND1) mitochondrial gene were optimized for the detection of Echinococcus shiquicus, Echinococcus granulosus G1, and Echinococcus multilocularis DNA derived from parasite tissue or canid fecal samples. Specificity using parasite...
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