Article
Functional identification of a hydroxyproline-o-galactosyltransferase specific for arabinogalactan protein biosynthesis in Arabidopsis.
The Journal of biological chemistry - 5 Apr 2013
Basu Debarati, Liang Yan, Liu Xiao, Himmeldirk Klaus, Faik Ahmed, Kieliszewski Marcia, Held Michael, Showalter Allan M
Abstract excerpt
Although plants contain substantial amounts of arabinogalactan proteins (AGPs), the enzymes responsible for AGP glycosylation are largely unknown. Bioinformatics indicated that AGP galactosyltransferases (GALTs) are members of the carbohydrate-active enzyme glycosyltransferase (GT) 31 family (CAZy GT31) involved in N- and O-glycosylation. Six Arabidopsis GT31 members were expressed in Pichia pastoris and tested...
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