Article
Construction of a primary DNA fingerprint database for cotton cultivars.
Genetics and molecular research : GMR - 13 Jun 2013
Zhang Y C, Kuang M, Yang W H, Xu H X, Zhou D Y, Wang Y Q, Feng X A, Su C, Wang F
Abstract excerpt
Forty core primers were used to construct a DNA fingerprint database of 132 cotton species based on multiplex fluorescence detection technology. A high first successful ratio of 99.04% was demonstrated with tetraplex polymerase chain reaction. Forty primer pairs amplified a total of 262 genotypes among 132 species, with an average of 6.55 per primer and values of polymorphism information content varying from...
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