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Development of microsatellite markers for the Neotropical endemic Brazilian Guanabara frog, Euparkerella brasiliensis, through 454 shotgun pyrosequencing.

Genetics and molecular research : GMR - 24 Jan 2013

Fusinatto L A, Lopes S, Silva-Ferreira A, Alexandrino J, Haddad C F B, Rocha C F D, Sequeira F

Abstract excerpt

The new-generation 454 GS-FLX Titanium pyrosequencing was used to isolate microsatellite markers for the Brazilian Guanabara frog, Euparkerella brasiliensis, an Atlantic forest endemic species. Three multiplex polymerase chain reaction sets were optimized for genotyping of 11 polymorphic (di- and tetranucleotide) microsatellite markers. Genetic diversity was assessed in 21 individuals from a population...

Topics

Alleles
Animals
Anura
Brazil
Genotype
Heterozygote
Linkage Disequilibrium
Microsatellite Repeats
Multiplex Polymerase Chain Reaction
Sequence Analysis, DNA

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PMID: 23408409
DOI: 10.4238/2013.January.24.15

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