Article
[Purification and some properties of electrophoretic variants of 6-phosphogluconate dehydrogenase from rat erythrocytes].
Biokhimiia (Moscow, Russia) - 1 Jan 2000
Serov O L, Manchenko G P, Khlebodarova T M
Abstract excerpt
Purification procedure of electrophoretic variants FF and SS of 6-phosphogluconate dehydrogenase (6-PGD) is described. The method includes (NH4)2SO4 fractionation and chromatography on DEAE- and CM-celluloses. Isoenzymes were purified about 5000 fold, and were found to be homogenous by disc electrophoresis in 7% polyacrylamide gel. It was found by comparative studies of activities of variants FF and SS, that pH...
Topics
- Animals
- Electrophoresis, Disc
- Erythrocytes
- Genetic Variation
- Hydrogen-Ion Concentration
- Isoenzymes
- Kinetics
- Phosphogluconate Dehydrogenase
- Rats