Article
Identification of Tunisian Leishmania spp. by PCR amplification of cysteine proteinase B (cpb) genes and phylogenetic analysis.
Acta tropica - 1 Mar 2013
Chaouch Melek, Fathallah-Mili Akila, Driss Mehdi, Lahmadi Ramzi, Ayari Chiraz, Guizani Ikram, Ben Said Moncef, Benabderrazak Souha
Abstract excerpt
Discrimination of the Old World Leishmania parasites is important for diagnosis and epidemiological studies of leishmaniasis. We have developed PCR assays that allow the discrimination between Leishmania major, Leishmania tropica and Leishmania infantum Tunisian species. The identification was performed by a simple PCR targeting cysteine protease B (cpb) gene copies. These PCR can be a routine molecular biology...
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