Article
Specific properties of enteropathogenic Escherichia coli isolates from diarrheal patients and comparison to strains from foods and fecal specimens from cattle, swine, and healthy carriers in Osaka City, Japan.
Applied and environmental microbiology - 1 Feb 2013
Wang Lili, Wakushima Mitsuko, Aota Tetsu, Yoshida Yuka, Kita Toshimasa, Maehara Tomofumi, Ogasawara Jun, Choi Changsun, Kamata Yoichi, Hara-Kudo Yukiko, Nishikawa Yoshikazu
Abstract excerpt
For exhaustive detection of diarrheagenic Escherichia coli, we previously developed a colony-hybridization method using hydrophobic grid-membrane filters in combination with multiplex real-time PCR. To assess the role of domestic animals as the source of atypical enteropathogenic E. coli (aEPEC), a total of 679 samples (333 from foods, fecal samples from 227 domestic animals, and 119 from healthy people) were...
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